Once the clone is constructed, GeneCopoeia follows additional quality control processes to ensure the right clone is delivered. Rigorous quality control and assurance processĪll ORF cDNA clones in the OmicsLink collection were generated from sequence validated full-length cDNA clones or high-quality human tissue cDNA libraries to construct expression-ready clones. See the list of reporter and affinity ligands available from GeneCopoeia. A collection of 20,000 human and 15,000 mouse genes may be ordered with HaloTag®.Ĭovalent and specific binding of a variety of synthetic reporter and affinity ligands to HaloTag® fusion proteins allows detection and affinity-binding or solid-phase fixation of tagged proteins. GeneCopoeia offers HaloTag® technology in a variety of mammalian and lentiviral expression vectors with the CMV promoter and neomycin selection marker. coli) and various eukaryotic cells.Ĭombined with OmicsLink™ expression-ready clones The 34 kDa monomeric protein can be fused at either the N-or C-terminus to proteins of interest and enables expression in both prokaryotic (E. The HaloTag® protein, a genetically engineered derivative of a dehalogenase, efficiently forms a covalent bond with various synthetic HaloTag® ligands. HaloTag® also enhances protein expression and solubility in addition to facilitating efficient protein purification.Thus, the HaloTag® can be used for a variety of downstream applications. This multi-functional tag binds covalently and specifically to a variety of synthetic ligands enabling tagged proteins to be labeled with fluorophores for both in vitro and in vivo imaging or with affinity agents for purification. The HaloTag® is the preferred system for multicolor cell imaging experiments with either live or fixed cells.
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